X-ray photoemission study of NiS_{2-x}Se_x (x = 0.0 - 1.2)

Electronic structure of NiS_{2-x}Se_x system has been investigated for various compositions (x) using x-ray photoemission spectroscopy. An analysis of the core level as well as the valence band spectra of NiS_2 in conjunction with many-body cluster calculations provides a quantitative description of the electronic structure of this compound. With increasing Se content, the on-site Coulomb correlation strength (U) does not change, while the band width W of the system increases, driving the system from a covalent insulating state to a pd-metallic state.Comment: 19 pages, 6 figures, To appear in Phys. Rev. B, 200

Decoupling Cache Coherence From XML in Model Checking

Many electrical engineers would agree that, had it not been for link-level acknowledgements, the eval- uation of congestion control might never have occurred. After years of significant research into Smalltalk, we validate the visualization of vacuum tubes. Our focus in our research is not on whether the famous encrypted algorithm for the visualization of simulated annealing by Garcia [5] is impossible, but rather on describing a novel application for the investigation of Lamport clocks (LealEst)

Experimental Study on Chamber Oscillations of Slotted Breakwater

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

Velocity slice imaging for dissociative electron attachment

A velocity slice imaging method is developed for measuring the angular distribution of fragment negative ions arising from dissociative electron attachment (DEA) to molecules. A low energy pulsed electron gun, a pulsed field ion extraction, and a two-dimensional position sensitive detector consisting of microchannel plates and a wedge-and-strip anode are used for this purpose. Detection and storage of each ion separately for its position and flight time allows analysis of the data offline for any given time slice, without resorting to pulsing the detector bias. The performance of the system is evaluated by measuring the angular distribution of O− from O2 and comparing it with existing data obtained using conventional technique. The capability of this technique in obtaining forward and backward angular distribution data is shown to have helped in resolving one of the existing problems in the electron scattering on O2

The release of inhibition model reproduces kinetics and plasticity of neurotransmitter release in central synapses

Calcium-evoked release of neurotransmitters from synaptic vesicles (SVs) is catalysed by SNARE proteins. The predominant view is that, at rest, complete assembly of SNARE complexes is inhibited ('clamped') by synaptotagmin and complexin molecules. Calcium binding by synaptotagmins releases this fusion clamp and triggers fast SV exocytosis. However, this model has not been quantitatively tested over physiological timescales. Here we describe an experimentally constrained computational modelling framework to quantitatively assess how the molecular architecture of the fusion clamp affects SV exocytosis. Our results argue that the 'release-of-inhibition' model can indeed account for fast calcium-activated SV fusion, and that dual binding of synaptotagmin-1 and synaptotagmin-7 to the same SNARE complex enables synergistic regulation of the kinetics and plasticity of neurotransmitter release. The developed framework provides a powerful and adaptable tool to link the molecular biochemistry of presynaptic proteins to physiological data and efficiently test the plausibility of calcium-activated neurotransmitter release models

Inhibition of Dermatophyte Fungi by Australian Jarrah Honey.

Superficial dermatophyte infections, commonly known as tineas, are the most prevalent fungal ailment and are increasing in incidence, leading to an interest in alternative treatments. Many floral honeys possess antimicrobial activity due to high sugar, low pH, and the production of hydrogen peroxide (H2O2) from the activity of the bee-derived enzyme glucose oxidase. Australian jarrah (Eucalyptus marginata) honey produces particularly high levels of H2O2 and has been found to be potently antifungal. This study characterized the activity of jarrah honey on fungal dermatophyte species. Jarrah honey inhibited dermatophytes with minimum inhibitory concentrations (MICs) of 1.5-3.5% (w/v), which increased to ≥25% (w/v) when catalase was added. Microscopic analysis found jarrah honey inhibited the germination of Trichophyton rubrum conidia and scanning electron microscopy of mature T. rubrum hyphae after honey treatment revealed bulging and collapsed regions. When treated hyphae were stained using REDOX fluorophores these did not detect any internal oxidative stress, suggesting jarrah honey acts largely on the hyphal surface. Although H2O2 appears critical for the antifungal activity of jarrah honey and its action on fungal cells, these effects persisted when H2O2 was eliminated and could not be replicated using synthetic honey spiked with H2O2, indicating jarrah honey contains agents that augment antifungal activity

Timing Offset Calibration of CZTI instrument aboard ASTROSAT

The radio as well as the high energy emission mechanism in pulsars is yet not understood properly. A multi-wavelength study is likely to help in better understanding of such processes. The first Indian space-based observatory, ASTROSAT, has five instruments aboard, which cover the electromagnetic spectrum from infra-red (1300 $\AA$) to hard X-ray (380 KeV). Cadmium Zinc Telluride Imager (CZTI), one of the five instruments is a hard X-ray telescope functional over an energy range of 20-380 KeV. We aim to estimate the timing offset introduced in the data acquisition pipeline of the instrument, which will help in time alignment of high energy time series with those from two other ground-based observatories, viz. the Giant Meterwave Radio Telescope (GMRT) and the Ooty Radio Telescope (ORT). PSR B0531+21 is a well-studied pulsar with nearly aligned radio and hard X-ray pulse profiles. We use simultaneous observations of this pulsar with the ASTROSAT, the ORT and the GMRT. The pulsar was especially observed using the ORT with almost daily cadence to obtain good timing solutions. We also supplement the ORT data with archival FERMI data for estimation of timing noise. The timing offset of ASTROSAT instruments was estimated from fits to arrival time data at the ASTROSAT and the radio observatories. We estimate the offset between the GMRT and the ASTROSAT-CZTI to be -4716 $\pm$ 50 $\mu s$. The corresponding offset with the ORT was -29639 $\pm$ 50 $\mu s$. The offsets between the GMRT and Fermi-LAT -5368 $\pm$ 56 $\mu s$. (Abridged)Comment: 6 pages, 5 figures, 2 tables, Revised and Updated, accepted for publication in A&

Multi-tasking Sulf1/Sulf2 enzymes do not only facilitate extracellular cell signalling but also participate in cell cycle related nuclear events

This study demonstrates highly dynamic spatial and temporal pattern of SULF1/SULF2 expression in a number of neuronal cell types growing in normal culture medium that included their transient nuclear mobilisation. Their nuclear translocation became particularly apparent during cell proliferation as both SULF1/SULF2 demonstrated not only cell membrane associated expression, their known site of function but also transient nuclear mobilisation during nuclear cell division. Nuclear localisation was apparent not only by immunocytochemical staining but also confirmed by immunoblotting staining of isolated nuclear fractions of C6, U87 and N2A cells. Immunocytochemical analysis demonstrated rapid nuclear exit of both SULF1/SULF2 following cell division that was slightly delayed but not blocked in a fraction of the polyploid cells observed in C6 cells. The overexpression of both Sulf1 and Sulf2 genes in C6 and U87 cells markedly promoted in vitro growth of these cells accompanied by nuclear mobilisation while inhibition of both these genes inhibited cell proliferation with little or no nuclear SULF1/SULF2 mobilisation. SULF1/SULF2 activity in these cells thus demonstrated a clear co-ordination of extracellular cell signalling with nuclear events related to cell proliferation

CdO-based nanostructures as novel CO2 gas sensors

Crystalline Cd(OH)2/CdCO3 nanowires, having lengths in the range from 0.3 up to several microns and 5–30 nm in diameter, were synthesized by a microwave-assisted wet chemical route and used as a precursor to obtain CdO nanostructures after a suitable thermal treatment in air. The morphology and microstructure of the as-synthesized and annealed materials have been investigated by scanning electron microscopy, transmission electron microscopy, x-ray diffraction and thermogravimetry–differential scanning calorimetry. The change in morphology and electrical properties with temperature has revealed a wire-to-rod transformation along with a decreases of electrical resistance. Annealed samples were printed on a ceramic substrate with interdigitated contacts to fabricate resistive solid state sensors. Gas sensing properties were explored by monitoring CO2 in synthetic air in the concentration range 0.2–5 v/v% (2000–50 000 ppm). The effect of annealing temperature, working temperature and CO2 concentration on sensing properties (sensitivity, response/recovery time and stability) were investigated. The results obtained demonstrate that CdO-based thick films have good potential as novel CO2 sensors for practical applications

Drought tolerance in MnSOD transgenic Hevea brasiliensis in a dry sub-humid environment

One year old bud-grafted plants of MnSOD transgenic Hevea lines (L1 and L2) and an untransformed line of clone RRII 105 were used in the present study to evaluate their physiological performance in a dry sub-humid environment by withholding irrigation and to assess the recovery by re-watering. The dry matter partitioning was more towards the root in transgenic lines (55% and 60% in Ll and L2, respectively) but, was less in the untransformed RRII 105 (43%). After six days of moisture stress in polybags, pre-dawn leaf water potential and relative water content declined in all the lines, however, transgenic line L1 showed higher tissue water content throughout the drought as well as recovery period. Chlorophyll content did not show any significant reduction. Net photosynthetic rate (PN) declined rapidly and it reached near zero on the third day of drought imposition except for line L1, which showed lesser decline in PN. The decline in stomatal conductance (gs) was more rapid than PN in all the lines. On re-watering, recovery of PN and gs was better in the transgenic lines than untransformed RRII 105, which did not recover fully from the drought impact. Antioxidant enzymes, superoxide dismutase and peroxidase did not show a definite trend in their activities in these lines. However, it was found that the transgenic line L1 had better drought tolerant capacity in terms of lesser inhibition of photosynthetic rate under drought and faster recovery on re-watering